This comprehensive yet balanced work emphasizes the principles and rationale underlying recombinant DNA methodology while furnishing a general understanding of the experimental protocols-suggesting flexible approaches to resolving particular molecular necessities that are easily adaptable to readers'' specific applications.
Features summary tables presenting at-a-glance information on practices of recombinant DNA methodologies!

Recombinant DNA Principles and Methodologies discusses basic and advanced topics requisite to the employment of recombinant DNA technology, such as

• plasmid biology
• nucleic acid biochemistry
• restriction enzymes
• cloning strategies
• gel electrophoresis
• southern and northern blotting
• preparation of probes
• phage lambda biology
• cosmids and genome analysis
• cloned gene expression
• polymerase chain reaction
• conventional and automated DNA sequencing
• site-directed mutagenesis
• and more!

Elucidating the material with over 2250 edifying references, equations, drawings, and photographs, this state-of-the-art resource is a valuable hands-on guide for molecular and cell biologists, biochemists, bioprocess technologists, applied and industrial microbiologists, virologists, geneticists, chemical engineers, and upper-level undergraduate and graduate students in these disciplines.