Correlative Light and Electron MIcroscopy

Introduction to CLEM

The combination of electron microscopy with transmitted light microscopy (termed correlative light and electron microscopy; CLEM) has been employed for decades to generate molecular identification that can be visualized by a dark, electron-dense precipitate.

Contents of the Volume

This new volume of Methods in Cell Biology covers many areas of CLEM, including a brief history and overview on CLEM methods, imaging of intermediate stages of meiotic spindle assembly in C. elegans embryos using CLEM, and capturing endocytic segregation events with HPF-CLEM.

Highlights

- Covers many areas of CLEM by the best international scientists in the field

- Includes a brief history and overview on CLEM methods