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Circulating Tumor Cells
Methods and Protocols
Introduction
This second edition explores new and updated approaches for enrichment, detection, isolation, and molecular profiling of circulating tumor cells (CTCs).
Chapter Content
Chapters detail comprehensive protocols for the capture of CTCs via filtration, density gradient centrifugation, microfluidic, immunomagnetic separation of CTCs, detection of CTCs by immunocytochemistry, fluorescence in situ hybridization, flow cytometry, assays designed for genomic characterization, and functional analyses of CTCs.
Format and Features
Written in the highly successful Methods in Molecular Biology series format, the chapters include brief introductions to the material, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section which highlights tips on troubleshooting and avoiding known pitfalls.
Objective
Authoritative and cutting-edge, Circulating Tumor Cells: Methods and Protocols, Second Edition aims to be a comprehensive guide for researchers in the field.
