Overview

Tissue Culture: Methods and Applications presents an overview of the procedures for working with cells in culture and for using them in a wide variety of scientific disciplines.

Content Highlights

The book discusses primary tissue dissociation; the preparation of primary cultures; cell harvesting; and replicate culture methods. The text also describes protocols on single cell isolations and cloning; perfusion and mass culture techniques; cell propagation on miscellaneous culture supports; and the evaluation of culture dynamics.

Advanced Techniques

The recent techniques facilitating microscopic observation of cells; cell hybridization; and virus propagation and assay are also encompassed.

Additional Topics

The book further tackles the production of hormones and intercellular substances; the diagnosis and understanding of disease; as well as quality control measures.

Target Audience

Scientists and professionals interested in methodology per se will find the book invaluable.