£63.99
Mycobacteria Protocols
Mycobacteria and Their Importance
Mycobacteria include species of great medical importance, being responsible for both human and animal disease, including tuberculosis and lep- .
Advances in Molecular Techniques
The use of molecular techniques to explore the biology of these organisms has exploded in the past few years.
Laboratory Challenges
Mycobacteria pose particular problems in the laboratory, most notably their slow growth rate, their tendency to clump, and their resistance to standard methods for lysis; thus special adaptations of provides - commonly used methods are required.
Protocols and Techniques
Mycobacteria Protocols scriptions of both basic and recently developed techniques for studying the molecular biology of mycobacteria. Although many genetic tools are still required, there is much that can be accomplished using those available, and this book provides a starting point for anyone interested in working on these intriguing, but difficult bacteria.
Basic Techniques
The first eight chapters describe the basic techniques required for any study of mycobacteria, including culture and safety aspects, and preparation of nucleic acids, proteins, and cell-wall fractions.
Genetic System Development
The introduction of DNA into mycobacteria was a major advance in enabling the development of genetic systems. Two chapters deal with introduction of DNA by electroporation and conjugation.
Use of Phages and Protocols
Phages have also been used as the basis for genetic systems, and a comprehensive list of protocols for their use is presented.
Current Challenges
It is becoming difficult to handle the rapidly increasing body of knowledge in the mycobacterial field.
