Methods of Enzymatic Analysis, Volume 2

Reviews developments in the determination of enzyme activity, including advances in assay techniques. It discusses the principles on which measurements of enzymes are based, with each chapter including equations and each method consisting of the pipetting protocol.

This volume is divided into four parts, each discussing a group of enzymes and their determination.

Part I: Oxidoreductases

Such as sorbitol dehydrogenase, lactate dehydrogenase, malate dehydrogenase, isocitrate dehydrogenase, 6-phosphogluconate dehydrogenase, xanthine oxidase, and glutamate dehydrogenase.

Part II: Transferases

Ranging from ornithine carbamoyltransferase and transamidinase to transketolase, transaldolase, UDP-glucuronyltransferase, glutamate-pyruvate transaminase, and phosphotransferases.

Part III: Hydrolases

Including esterases, glycoside hydrolases, peptidases, and proteinases.

Part IV: Lyases, Isomerases, and Ligases

Such as fructose-1,6-diphosphate aldolase, 1-phosphofructoaldolase, glucosephosphate isomerase, and tetrahydrofolate formylase.

This book is a valuable resource for biochemists as well as students and researchers working in the field of analytical biochemistry.