Introduction

Showing chemists how to predict ion-exchange chromatography (IEC) separation behaviour and how to determine appropriate operating conditions, this reference illustrates procedures, apparatus and types of ion exchanges, emphasizing the design and application of large-scale IEC.

Features

Complete with more than 100 useful tables and diagrams, this text explains the effects of each variable on separation behaviour; compares models and equations describing behaviour of proteins in IEC with experimental results; introduces design calculation procedures for scale up, adapting IEC for individual requirements; discusses parameters affecting separation behaviour, including adsorption equilibria, stationary phase diffusion and axial dispersion; provides examples of applications, such as high or medium performance IEC and large scale operations and reviews both the theoretical and experimental literature.

Intended Audience

This text serves as a reference for analytical, agricultural, food, and pharmaceutical chemists, chromatographers, food scientists and technologists, biochemists and biotechnologists.