This book constitutes the refereed proceedings of the 13th International Conference on Combinatorics on Words, WORDS 2021, held virtually in September 2021.

The 14 revised full papers presented in this book together with 2 invited talks were carefully reviewed and selected from 18 submissions. WORDS is the main conference series devoted to the mathematical theory of words. In particular, the combinatorial, algebraic and algorithmic aspects of words are emphasized. Motivations may also come from other domains such as theoretical computer science, bioinformatics, digital geometry, symbolic dynamics, numeration systems, text processing, number theory, etc.